Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.
Identifieur interne : 000675 ( Main/Exploration ); précédent : 000674; suivant : 000676Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.
Auteurs : Shutoku Matsuyama [Japon] ; Naganori Nao [Japon] ; Kazuya Shirato [Japon] ; Miyuki Kawase [Japon] ; Shinji Saito [Japon] ; Ikuyo Takayama [Japon] ; Noriyo Nagata [Japon] ; Tsuyoshi Sekizuka [Japon] ; Hiroshi Katoh [Japon] ; Fumihiro Kato [Japon] ; Masafumi Sakata [Japon] ; Maino Tahara [Japon] ; Satoshi Kutsuna [Japon] ; Norio Ohmagari [Japon] ; Makoto Kuroda [Japon] ; Tadaki Suzuki [Japon] ; Tsutomu Kageyama [Japon] ; Makoto Takeda [Japon]Source :
- Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : ARN viral, Serine endopeptidases.
- virologie : Infections à coronavirus, Pneumopathie virale.
- Animaux, Cellules Vero, Culture virale, Flambées de maladies, Humains, Lignée cellulaire, Pandémies.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : RNA, Viral, Serine Endopeptidases.
- isolation & purification : Betacoronavirus.
- virology : Coronavirus Infections, Pneumonia, Viral.
- Animals, Cell Line, Chlorocebus aethiops, Disease Outbreaks, Humans, Pandemics, Vero Cells, Virus Cultivation.
Abstract
A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.
DOI: 10.1073/pnas.2002589117
PubMed: 32165541
Affiliations:
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Kantō</region></placeName></affiliation></author><author><name sortKey="Sakata, Masafumi" sort="Sakata, Masafumi" uniqKey="Sakata M" first="Masafumi" last="Sakata">Masafumi Sakata</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Tahara, Maino" sort="Tahara, Maino" uniqKey="Tahara M" first="Maino" last="Tahara">Maino Tahara</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology 3, 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Kantō</region></placeName></affiliation></author><author><name sortKey="Suzuki, Tadaki" sort="Suzuki, Tadaki" uniqKey="Suzuki T" first="Tadaki" last="Suzuki">Tadaki Suzuki</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kageyama, Tsutomu" sort="Kageyama, Tsutomu" uniqKey="Kageyama T" first="Tsutomu" last="Kageyama">Tsutomu Kageyama</name><affiliation wicri:level="1"><nlm:affiliation>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Influenza Virus 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Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan; matuyama@nih.go.jp mtakeda@nih.go.jp.</nlm:affiliation><country wicri:rule="url">Japon</country><wicri:regionArea>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Nao, Naganori" sort="Nao, Naganori" uniqKey="Nao N" first="Naganori" last="Nao">Naganori Nao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Shirato, Kazuya" sort="Shirato, Kazuya" uniqKey="Shirato K" first="Kazuya" last="Shirato">Kazuya Shirato</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kawase, Miyuki" sort="Kawase, Miyuki" uniqKey="Kawase M" first="Miyuki" last="Kawase">Miyuki Kawase</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Saito, Shinji" sort="Saito, Shinji" uniqKey="Saito S" first="Shinji" last="Saito">Shinji Saito</name><affiliation wicri:level="1"><nlm:affiliation>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Takayama, Ikuyo" sort="Takayama, Ikuyo" uniqKey="Takayama I" first="Ikuyo" last="Takayama">Ikuyo Takayama</name><affiliation wicri:level="1"><nlm:affiliation>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Nagata, Noriyo" sort="Nagata, Noriyo" uniqKey="Nagata N" first="Noriyo" last="Nagata">Noriyo Nagata</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Sekizuka, Tsuyoshi" sort="Sekizuka, Tsuyoshi" uniqKey="Sekizuka T" first="Tsuyoshi" last="Sekizuka">Tsuyoshi Sekizuka</name><affiliation wicri:level="1"><nlm:affiliation>Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Katoh, Hiroshi" sort="Katoh, Hiroshi" uniqKey="Katoh H" first="Hiroshi" last="Katoh">Hiroshi Katoh</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kato, Fumihiro" sort="Kato, Fumihiro" uniqKey="Kato F" first="Fumihiro" last="Kato">Fumihiro Kato</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Sakata, Masafumi" sort="Sakata, Masafumi" uniqKey="Sakata M" first="Masafumi" last="Sakata">Masafumi Sakata</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Tahara, Maino" sort="Tahara, Maino" uniqKey="Tahara M" first="Maino" last="Tahara">Maino Tahara</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kutsuna, Satoshi" sort="Kutsuna, Satoshi" uniqKey="Kutsuna S" first="Satoshi" last="Kutsuna">Satoshi Kutsuna</name><affiliation wicri:level="1"><nlm:affiliation>Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo 162-8655, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo 162-8655</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Ohmagari, Norio" sort="Ohmagari, Norio" uniqKey="Ohmagari N" first="Norio" last="Ohmagari">Norio Ohmagari</name><affiliation wicri:level="1"><nlm:affiliation>Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo 162-8655, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo 162-8655</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kuroda, Makoto" sort="Kuroda, Makoto" uniqKey="Kuroda M" first="Makoto" last="Kuroda">Makoto Kuroda</name><affiliation wicri:level="1"><nlm:affiliation>Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Suzuki, Tadaki" sort="Suzuki, Tadaki" uniqKey="Suzuki T" first="Tadaki" last="Suzuki">Tadaki Suzuki</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kageyama, Tsutomu" sort="Kageyama, Tsutomu" uniqKey="Kageyama T" first="Tsutomu" last="Kageyama">Tsutomu Kageyama</name><affiliation wicri:level="1"><nlm:affiliation>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Influenza Virus Research Center, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Takeda, Makoto" sort="Takeda, Makoto" uniqKey="Takeda M" first="Makoto" last="Takeda">Makoto Takeda</name><affiliation wicri:level="1"><nlm:affiliation>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640, Japan; matuyama@nih.go.jp mtakeda@nih.go.jp.</nlm:affiliation><country wicri:rule="url">Japon</country><wicri:regionArea>Department of Virology 3, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="eISSN">1091-6490</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Betacoronavirus (isolation & purification)</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Coronavirus Infections (virology)</term><term>Disease Outbreaks</term><term>Humans</term><term>Pandemics</term><term>Pneumonia, Viral (virology)</term><term>RNA, Viral (metabolism)</term><term>Serine Endopeptidases (metabolism)</term><term>Vero Cells</term><term>Virus Cultivation</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (métabolisme)</term><term>Animaux</term><term>Cellules Vero</term><term>Culture virale</term><term>Flambées de maladies</term><term>Humains</term><term>Infections à coronavirus (virologie)</term><term>Lignée cellulaire</term><term>Pandémies</term><term>Pneumopathie virale (virologie)</term><term>Serine endopeptidases (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Viral</term><term>Serine Endopeptidases</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Betacoronavirus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN viral</term><term>Serine endopeptidases</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term><term>Pneumopathie virale</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Disease Outbreaks</term><term>Humans</term><term>Pandemics</term><term>Vero Cells</term><term>Virus Cultivation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Culture virale</term><term>Flambées de maladies</term><term>Humains</term><term>Lignée cellulaire</term><term>Pandémies</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name></region><name sortKey="Kageyama, Tsutomu" sort="Kageyama, Tsutomu" uniqKey="Kageyama T" first="Tsutomu" last="Kageyama">Tsutomu Kageyama</name><name sortKey="Kato, Fumihiro" sort="Kato, Fumihiro" uniqKey="Kato F" first="Fumihiro" last="Kato">Fumihiro Kato</name><name sortKey="Katoh, Hiroshi" sort="Katoh, Hiroshi" uniqKey="Katoh H" first="Hiroshi" last="Katoh">Hiroshi Katoh</name><name sortKey="Kawase, Miyuki" sort="Kawase, Miyuki" uniqKey="Kawase M" first="Miyuki" last="Kawase">Miyuki Kawase</name><name sortKey="Kuroda, Makoto" sort="Kuroda, Makoto" uniqKey="Kuroda M" first="Makoto" last="Kuroda">Makoto Kuroda</name><name sortKey="Kutsuna, Satoshi" sort="Kutsuna, Satoshi" uniqKey="Kutsuna S" first="Satoshi" last="Kutsuna">Satoshi Kutsuna</name><name sortKey="Nagata, Noriyo" sort="Nagata, Noriyo" uniqKey="Nagata N" first="Noriyo" last="Nagata">Noriyo Nagata</name><name sortKey="Nao, Naganori" sort="Nao, Naganori" uniqKey="Nao N" first="Naganori" last="Nao">Naganori Nao</name><name sortKey="Ohmagari, Norio" sort="Ohmagari, Norio" uniqKey="Ohmagari N" first="Norio" last="Ohmagari">Norio Ohmagari</name><name sortKey="Saito, Shinji" sort="Saito, Shinji" uniqKey="Saito S" first="Shinji" last="Saito">Shinji Saito</name><name sortKey="Sakata, Masafumi" sort="Sakata, Masafumi" uniqKey="Sakata M" first="Masafumi" last="Sakata">Masafumi Sakata</name><name sortKey="Sekizuka, Tsuyoshi" sort="Sekizuka, Tsuyoshi" uniqKey="Sekizuka T" first="Tsuyoshi" last="Sekizuka">Tsuyoshi Sekizuka</name><name sortKey="Shirato, Kazuya" sort="Shirato, Kazuya" uniqKey="Shirato K" first="Kazuya" last="Shirato">Kazuya Shirato</name><name sortKey="Suzuki, Tadaki" sort="Suzuki, Tadaki" uniqKey="Suzuki T" first="Tadaki" last="Suzuki">Tadaki Suzuki</name><name sortKey="Tahara, Maino" sort="Tahara, Maino" uniqKey="Tahara M" first="Maino" last="Tahara">Maino Tahara</name><name sortKey="Takayama, Ikuyo" sort="Takayama, Ikuyo" uniqKey="Takayama I" first="Ikuyo" last="Takayama">Ikuyo Takayama</name><name sortKey="Takeda, Makoto" sort="Takeda, Makoto" uniqKey="Takeda M" first="Makoto" last="Takeda">Makoto Takeda</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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